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Nat Methods. 2007 Apr;4(4):323-6. Epub 2007 Mar 18.

Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.

Author information

1
Department of Physiology, University of California, San Francisco, California 94158, USA.

Abstract

We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.

PMID:
17369834
DOI:
10.1038/nmeth1033
[Indexed for MEDLINE]

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