Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line

Nadine Platet; Jean-François Mayol; François Berger; Francis Hérodin; Didier Wion

doi:10.1016/j.febslet.2007.02.071

Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line

FEBS Lett. 2007 Apr 3;581(7):1435-40. doi: 10.1016/j.febslet.2007.02.071. Epub 2007 Mar 7.

Authors

Nadine Platet¹, Jean-François Mayol, François Berger, Francis Hérodin, Didier Wion

Affiliation

¹ INSERM U318, CHU Michallon, 38043 Grenoble, France.

PMID: 17362939
DOI: 10.1016/j.febslet.2007.02.071

Abstract

Using the C6 glioma cell as a paradigm, we found that (i) the clonogenicity of C6 cells is several orders of magnitude higher than the percentage of SP cells; (ii) non-SP cells are able to generate SP cells, and conversely SP cells generate non-SP cells; (iii) non-SP sorted cells behave as tumorigenic cells. Hence, in C6 cells cultured in serum-containing medium, SP cells can be generated from non-SP cells. This dynamic equilibrium explains in C6 cells the maintenance of the SP phenotype with cell passaging and demonstrates the existence of tumorigenic non-SP cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters / analysis
ATP-Binding Cassette Transporters / metabolism
Animals
Cell Line, Tumor / metabolism
Cell Line, Tumor / pathology*
Cell Transformation, Neoplastic / metabolism
Cell Transformation, Neoplastic / pathology*
Clone Cells / metabolism
Clone Cells / pathology
Glioma / metabolism
Glioma / pathology*
Phenotype
Rats

Substances

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters
Abcg2 protein, rat