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Intervirology. 2007;50(3):224-8. Epub 2007 Mar 14.

HIV Tat protein increases Bcl-2 expression in monocytes which inhibits monocyte apoptosis induced by tumor necrosis factor-alpha-related apoptosis-induced ligand.

Author information

1
Department of Infectious Diseases, The First Affiliated Hospital, Medical School, Key Laboratory of Infectious Diseases of Chinese Ministry of Public Health, Zhejiang University, Hangzhou, Zhejiang, PR China.

Abstract

OBJECTIVE:

To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL).

METHODS:

Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexin V and 7-AAD staining were used to detect apoptosis of monocytes induced by TRAIL.

RESULTS:

HIV Tat protein increased Bcl-2 expression in human monocytes in a dose-dependent manner. Annexin V staining showed that 51.54% of monocytes underwent apoptosis after being treated with 100 ng/ml recombinant TRAIL. When monocytes were prestimulated with HIV Tat, only 15.46% of monocytes underwent apoptosis. This effect can be inhibited by polyclonal anti-Tat serum. 7-AAD staining showed similar results.

CONCLUSION:

HIV Tat protein increases Bcl-2 expression in monocytes which inhibited apoptosis induced by TRAIL. HIV Tat protein may play an important role in the mechanisms of HIV-persistent infection in monocytes.

PMID:
17356300
PMCID:
PMC2384232
DOI:
10.1159/000100565
[Indexed for MEDLINE]
Free PMC Article

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