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Methods Enzymol. 2007;421:126-40.

Creating recombination-activated genes and sequence-defined mutant libraries using transposons.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Abstract

The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.

PMID:
17352920
DOI:
10.1016/S0076-6879(06)21012-7
[Indexed for MEDLINE]

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