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Nat Biotechnol. 2007 Apr;25(4):443-5. Epub 2007 Mar 11.

Reporter proteins for in vivo fluorescence without oxygen.

Author information

1
Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Research Center Juelich, Stetternicher Forst, D-52426 Juelich, Germany.

Abstract

Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.

PMID:
17351616
DOI:
10.1038/nbt1293
[Indexed for MEDLINE]

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