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Curr Genet. 1992 Jan;21(1):83-4.

One-step transformation of yeast in stationary phase.

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1
Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China.

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

PMID:
1735128
[Indexed for MEDLINE]
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