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J Bacteriol. 2007 May;189(9):3489-95. Epub 2007 Mar 9.

The Escherichia coli regulator of sigma 70 protein, Rsd, can up-regulate some stress-dependent promoters by sequestering sigma 70.

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1
School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom.

Abstract

The Escherichia coli Rsd protein forms complexes with the RNA polymerase sigma(70) factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative sigma(38) factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with sigma(70). Our experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor.

PMID:
17351046
PMCID:
PMC1855875
DOI:
10.1128/JB.00019-07
[Indexed for MEDLINE]
Free PMC Article
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