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Fertil Steril. 2007 Oct;88(4):952-6. Epub 2007 Mar 9.

Survival rate of human oocytes and pregnancy outcome after vitrification using slush nitrogen in assisted reproductive technologies.

Author information

1
Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University, College of Medicine, Seoul, Korea. tkyoon@cha.ac.kr

Abstract

OBJECTIVE:

To report the survival rate of oocytes and the rate of successful pregnancies obtained from super-rapid cooling of oocytes using slush nitrogen (SN(2)).

DESIGN:

Prospective clinical research.

SETTING:

A university-affiliated hospital.

PATIENT(S):

Twenty-eight infertile women who underwent 30 cycles of IVF-ET using previously vitrified oocytes.

INTERVENTION(S):

Oocytes were vitrified by super-rapid cooling using SN(2).

MAIN OUTCOME MEASURE(S):

Morphological normality of thawed oocytes and clinical outcome.

RESULT(S):

In 30 cycles of ovarian stimulation for IVF, 364 surplus oocytes from 28 patients were vitrified using SN(2). Three hundred two (85.1% +/- 2.9%) of the oocytes survived after warming. Fertilization and cleavage rates were 77.4% +/- 3.5% (168/218) and 94.3% +/- 2.1% (158/168), respectively. Thirteen pregnancies (43.3%) resulted from 30 uterine transfers of 120 embryos with an implantation rate of 14.2% (17/120). There were no differences between the pregnancy rate after vitrification/warming and that obtained from routine noncryopreserved oocytes.

CONCLUSION(S):

The present report suggests that super-rapid cooling may improve the clinical efficacy of human oocyte vitrification and may be a valuable tool for human assisted reproductive technologies.

[Indexed for MEDLINE]

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