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Biochem Biophys Res Commun. 2007 Apr 27;356(1):67-71. Epub 2007 Feb 27.

Lateral diffusion of TGF-beta type I receptor studied by single-molecule imaging.

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Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.


In this report, we investigated the lateral diffusion of transforming growth factor beta (TGF-beta) type I receptor (TbetaRI) in living cells by imaging and tracking individual green fluorescent protein tagged TbetaRI on the cell membrane. We found that when co-expressed with TGF-beta type II receptor (TbetaRII), the mobility of TbetaRI decreased significantly after TGF-beta1 stimulation. However, in the cells that had been depleted of cholesterol with Nystatin or methyl-beta-cyclodextrin, the diffusion rate of TbetaRI was not changed by TGF-beta1 treatment. Our observations suggest that membrane lipid-rafts provide an environment that facilitates the association of TbetaRI and TbetaRII for cell signaling.

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