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J Histochem Cytochem. 2007 Jul;55(7):687-700. Epub 2007 Mar 6.

Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry.

Author information

1
Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida, USA.

Abstract

This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

PMID:
17341475
DOI:
10.1369/jhc.6A7156.2007
[Indexed for MEDLINE]

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