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Nat Methods. 2007 Apr;4(4):311-3. Epub 2007 Mar 4.

High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy.

Author information

1
Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. verveer@mpi-dortmund.mpg.de

Abstract

We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.

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PMID:
17339847
DOI:
10.1038/nmeth1017
[Indexed for MEDLINE]

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