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Eur Biophys J. 2007 Jul;36(6):675-81. Epub 2007 Feb 28.

Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.

Author information

1
Department of Physics and Astronomy, Vrije Universiteit, de Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands.

Abstract

Tetrameric motor proteins of the Kinesin-5 family are essential for eukaryotic cell division. The microscopic mechanism by which Eg5, the vertebrate Kinesin-5, drives bipolar mitotic spindle formation remains unknown. Here we show in optical trapping experiments that full-length Eg5 moves processively and stepwise along microtubule bundles. Interestingly, the force produced by individual Eg5 motors typically reached only approximately 2 pN, one-third of the stall force of Kinesin-1. Eg5 typically detached from microtubules before stalling. This behavior may reflect a regulatory mechanism important for the role of Eg5 in the mitotic spindle.

PMID:
17333163
PMCID:
PMC1914257
DOI:
10.1007/s00249-007-0134-6
[Indexed for MEDLINE]
Free PMC Article

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