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Ann N Y Acad Sci. 2007 Mar;1099:34-9. Epub 2007 Mar 1.

Topologic investigation of the NCKX2 Na+/Ca2+-K+ exchanger alpha-repeats.

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Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, 3330 Hospital Drive, N.W. Calgary, Alberta, T2N 4N1, Canada.


Algorithms suggest that NCKX proteins consist of an N-terminal signal peptide and 11 transmembrane segments divided in two groups of 5 and 6, respectively, separated by a large cytoplasmic loop. This predicted topology places the NCKX alpha-repeats with the same orientation in the plasma membrane. Using thiol-specific drug treatment and site-directed disulfide mapping, we have investigated the orientation of the NCKX2 alpha-repeats. Our results suggest that the NCKX2 alpha-repeats have an antiparallel orientation in the plasma membrane. In addition, these experiments suggest that the alpha-repeats are found in close proximity in the mature configuration of the protein.

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