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Nat Methods. 2007 Mar;4(3):219-22. Epub 2007 Feb 25.

Imaging receptor microdomains on leukocyte subsets in live mice.

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Department of Medicine and Immunobiology Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York 10029, USA.


We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

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