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J Chromatogr A. 2007 Jun 1;1151(1-2):211-5. Epub 2007 Feb 4.

Counter-current chromatography based analysis of synergy in an anti-tuberculosis ethnobotanical.

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1
Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.

Abstract

The crude extract of an Alaskan ethnobotanical plant, Oplopanax horridus, was subjected to counter-current chromatography (CCC), and the selected active regions were evaluated for their synergistic effects with an in vitro model of anti-tubercular efficacy. CCC as a support-free high-resolution separation method was employed to preclude potential irreversible absorption to a solid stationary phase. The microplate Alamar blue assay and the isobole method were used to measure the biological activity and eliminate dose-response dependent errors, respectively. Using the combination of CCC, bioassay and isobole method, significant synergistic effects were observed. Among the entire polarity range, fractions with distribution constant between 0.44 and 0.81 showed the most synergistic enhancement with an increase in potency by 108% for the recombined fractions.

PMID:
17316661
PMCID:
PMC2533621
DOI:
10.1016/j.chroma.2007.01.127
[Indexed for MEDLINE]
Free PMC Article
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