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Arch Biochem Biophys. 1992 Feb 1;292(2):583-8.

Transport of 4-hydroxyphenylacetic acid in Klebsiella pneumoniae.

Author information

1
Departamento de Bioquímica y Biologiá Molecular IV, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

Abstract

Klebsiella pneumoniae M5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-HPA). This transport system has a Kt of 16.3 microM and a maximal velocity of 31.2 nmol/min (milligrams dry weight). The transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular ATP concentrations, and the 4-HPA binding activity has been detected in the crude shock extracts. All these observations indicate that 4-HPA uptake is an active transport which involves a periplasmic binding protein and it seems to be energized by phosphate bond energy.

PMID:
1731619
DOI:
10.1016/0003-9861(92)90034-t
[Indexed for MEDLINE]

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