Abstract
Recent work has demonstrated the enhancement of hormone-stimulated inositol trisphosphate formation in renal epithelial cells under conditions of glucosylceramide depletion. The role of glucosylceramide metabolism was explored further by exposing Madin-Darby canine kidney (MDCK) cells to the beta-glucosidase inhibitor conduritol B epoxide, which produced time-dependent and concentration-dependent increases in glucosylceramide levels and decreased bradykinin-stimulated inositol trisphosphate formation from isolated MDCK cell membranes. These data provide further support for an association between glucosylceramide levels and hormone-stimulated inositol trisphosphate formation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bradykinin / pharmacology*
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Cell Line
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Dogs
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Galactose / metabolism
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Glucosylceramides / metabolism
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Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology*
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Inositol / analogs & derivatives*
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Inositol / metabolism
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Inositol / pharmacology
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Inositol 1,4,5-Trisphosphate / metabolism*
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Kidney
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Kinetics
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Palmitic Acid
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Palmitic Acids / metabolism
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Radioisotope Dilution Technique
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Sphingolipids / metabolism
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Tritium
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beta-Glucosidase / antagonists & inhibitors*
Substances
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Glucosylceramides
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Palmitic Acids
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Sphingolipids
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Tritium
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Palmitic Acid
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Guanosine 5'-O-(3-Thiotriphosphate)
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Inositol
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Inositol 1,4,5-Trisphosphate
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beta-Glucosidase
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conduritol epoxide
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Bradykinin
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Galactose