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J Mol Biol. 1992 Jan 5;223(1):55-66.

Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10.

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Department of Molecular and Medical Genetics, University of Toronto, Canada.


The Escherichia coli proteins NusB and ribosomal protein S10 are important for transcription antitermination by the bacteriophage lambda N protein. We have used sucrose gradient co-sedimentation and affinity chromatography with immobilized ribosomal protein S10, a glutathione S-transferase-S10 fusion protein, and NusB to show that NusB binds directly and very selectively to S10. The interaction is non-ionic and has an estimated Kd value of 10(-7) M. We hypothesize that NusB binds to N-modified transcription complexes primarily by interacting with S10.

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