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J Biol Chem. 1992 Jan 15;267(2):975-8.

Sequence of a cDNA clone encoding pig heart mitochondrial CoA transferase.

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1
Department of Biochemistry, University of Alberta, Edmonton, Canada.

Abstract

We have isolated a full-length cDNA clone encoding the cytoplasmic precursor to pig heart mitochondrial CoA transferase (succinyl-CoA:3-ketoacid coenzyme A transferase (3-oxoacid CoA transferase, EC 2.8.3.5], a key enzyme for ketone body catabolism. The deduced amino acid sequence indicates the presence of a 39-residue mitochondrial signal sequence and a 481-residue mature protein of molecular weight 52,197. CoA transferase is known to be susceptible to proteolytic cleavage to produce a nicked but active enzyme. We have identified the site of proteolysis, and analysis of the sequence in its vicinity suggests that the polypeptide may fold into two domains connected by a highly hydrophilic bridge.

PMID:
1730685
[Indexed for MEDLINE]
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