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Bioconjug Chem. 2007 Mar-Apr;18(2):281-4. Epub 2007 Feb 14.

Poly(vinyl alcohol) functionalized poly(dimethylsiloxane) solid surface for immunoassay.

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1
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.

Abstract

In this communication, we describe a simple and robust method for the covalent bonding of poly(vinyl alcohol) (PVA) on a silanized poly(dimethylsiloxane) (PDMS) surface. Nonspecific adsorption of proteins via hydrophobic-hydrophobic interactions of the PVA-coated surface is greatly reduced, and biomolecules can be rapidly anchored on the PVA-coated surface with high loading and uniformity. On the basis of a sandwich immunoassay with the anti-rabbit IgG and IgG pair as a model, the detection limit for IgG is down to 1 pg/mL with linearity up to 11 microg the levels often encountered in biological, forensic, and environmental samples.

PMID:
17298027
DOI:
10.1021/bc060108p
[Indexed for MEDLINE]
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