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Arch Oral Biol. 2007 Jul;52(7):684-90. Epub 2007 Jan 31.

MEPE expression in osteocytes during orthodontic tooth movement.

Author information

1
Department of Orthodontics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. gluhak@uthscsa.edu

Abstract

MEPE and DMP1 may play a role in mineralisation and demineralisation within the osteocyte microenvironment. Our earlier studies showed that DMP1 is mechanically responsive [Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, et al. Mechanical loading stimulates dentin matrix protein 1 (DMP1) in osteocytes in vivo. J Bone Min Res 2003;18(5):807-17].

OBJECTIVES:

To examine the effect of mechanical loading on the expression of MEPE using mouse tooth movement model, and compare this effect to that on DMP1.

METHODS:

In situ hybridisation and immunohistochemistry was performed on 38 treated and 38 control bone sites loaded 6-72 h. ImageJ was used for quantification of mRNA expression in osteocytes.

RESULTS:

Alveolar osteocytes showed high basal level of MEPE that decreased during the first day of loading, followed by 2.8-fold stimulation at day 3, and returning to a control level by day 7.

CONCLUSION:

The osteocyte specific mechanical stimulation of MEPE was delayed and different, compared to that of DMP1. This suggests a distinct role of MEPE and DMP1 in the response of osteocytes to mechanical loading in vivo.

PMID:
17270144
PMCID:
PMC1868431
DOI:
10.1016/j.archoralbio.2006.12.010
[Indexed for MEDLINE]
Free PMC Article
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