Format

Send to

Choose Destination
Nat Biotechnol. 2007 Feb;25(2):217-9. Epub 2007 Jan 28.

Artificial reporter gene providing MRI contrast based on proton exchange.

Author information

1
The Russell H. Morgan Department of Radiology and Radiological Science, Neurosection, Division of MR Research, Traylor Building Rm. 217, 720 Rutland Ave., Baltimore, Maryland 21205, USA.

Abstract

Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

PMID:
17259977
DOI:
10.1038/nbt1277
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center