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Biochim Biophys Acta. 2007 Jan;1769(1):5-19. Epub 2006 Dec 29.

Molecular characterization of the Caenorhabditis elegans REF-1 family member, hlh-29/hlh-28.

Author information

1
Department of Biology, School of Computer, Mathematical, and Natural Sciences, Morgan State University, 1700 E. Coldspring Lane, Baltimore, MD 21251, USA.

Abstract

Members of the Caenorhabditis elegans REF-1 family of bHLH proteins are atypical in that each protein contains two bHLH domains. In this study we describe a functional and molecular characterization of the REF-1 family members, hlh-29/hlh-28. 5'-RACE results confirm the presence of two bHLH domain coding regions in a single transcript and quantitative PCR (qPCR) shows that hlh-29/hlh-28 mRNA is detected in wild-type animals throughout development. A promoter fusion of hlh-29 to the green fluorescent protein shows post-embryonic reporter activity in cells of the vulva, the somatic gonad, the intestine and in neuronal cells of the head and tail. Loss of hlh-29/hlh-28 function via RNA interference (RNAi) results in multiple phenotypes including late embryonic lethality, yolk protein accumulation, everted vulva, bordering behavior, and alter chemosensory responses.

PMID:
17258327
DOI:
10.1016/j.bbaexp.2006.12.001
[Indexed for MEDLINE]

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