Format

Send to

Choose Destination
Fungal Genet Biol. 2007 Oct;44(10):979-90. Epub 2006 Dec 16.

Involvement of Candida albicans pyruvate dehydrogenase complex protein X (Pdx1) in filamentation.

Author information

1
Department of Pediatrics and Program in Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520-8064, USA.

Abstract

For 50 years, physiologic studies in Candida albicans have associated fermentation with filamentation and respiration with yeast morphology. Analysis of the mitochondrial proteome of a C. albicans NDH51 mutant, known to be defective in filamentation, identified increased expression of several proteins in the respiratory pathway. Most notable was a 15-fold increase in pyruvate dehydrogenase complex protein X (Pdx1), an essential component of the pyruvate dehydrogenase complex. In basal salts medium with < or = 100 mM glucose as carbon source, two independent pdx1 mutants displayed a filamentation defect identical to ndh51; reintegration of one PDX1 allele restored filamentation. Concentrations of glucose < or = 100 mM did not correct the filamentation defect. Expanding on previous work, these studies suggest that increased expression of proteins extraneous to the electron transport chain compensates for defects in the respiratory pathway to maintain yeast morphology. Mitochondrial proteomics can aid in the identification of C. albicans genes not previously implicated in filamentation.

PMID:
17254815
PMCID:
PMC2062515
DOI:
10.1016/j.fgb.2006.12.003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center