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Antonie Van Leeuwenhoek. 1991 Oct-Nov;60(3-4):145-58.

Quantifying heterogeneity: flow cytometry of bacterial cultures.

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1
Department of Biological Sciences, University College of Wales, Aberystwyth, UK.

Abstract

Flow cytometry is a technique which permits the characterisation of individual cells in populations, in terms of distributions in their properties such as DNA content, protein content, viability, enzyme activities and so on. We review the technique, and some of its recent applications to microbiological problems. It is concluded that cellular heterogeneity, in both batch and continuous axenic cultures, is far greater than is normally assumed. This has important implications for the quantitative analysis of microbial processes.

PMID:
1725477
[Indexed for MEDLINE]

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