Format

Send to

Choose Destination
Planta Med. 1991 Jun;57(3):263-70.

HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates.

Author information

1
Murdock Healthcare, Springville, Utah 84663, U.S.A.

Abstract

Reversed-phase high Performance liquid chromatography (C18-HPLC) was used to separate and quantitate all the detectable alkyl and alkenyl thiosulfinates, including configurational isomers, of garlic homogenates. Pure thiosulfinates were synthesized or isolated and identified by (1)H-NMR, and their extinction coefficients determined. Some configurational isomers required Separation by silica-HPLC. Five previously unreported thiosulfinates have been found, four of which contain the TRANS-1-propenyl group and increase several-fold to over half the content of allicin upon storage of garlic bulbs at 4 degrees C with a concomitant decrease in a gamma-glutamyl peptide. The variation in thiosulfinate yield between different countries, stores, bulbs, cloves, and storage times was investigated. A method for standardizing the quantitation of allicin yield from garlic is proposed and compared to other methods of allicin analysis.

PMID:
17226157
DOI:
10.1055/s-2006-960087

Supplemental Content

Full text links

Icon for Georg Thieme Verlag Stuttgart, New York
Loading ...
Support Center