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PLoS One. 2007 Jan 17;2(1):e162.

High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells.

Author information

1
Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.

Abstract

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.

PMID:
17225864
PMCID:
PMC1764711
DOI:
10.1371/journal.pone.0000162
[Indexed for MEDLINE]
Free PMC Article

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