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PLoS One. 2006 Dec 20;1:e104.

Genome-wide reverse genetics framework to identify novel functions of the vertebrate secretome.

Author information

1
Department of Oral Sciences and Minnesota Craniofacial Research Training Program (MinnCResT), University of Minnesota, Minneapolis, Minnesota, United States of America.

Abstract

BACKGROUND:

Understanding the functional role(s) of the more than 20,000 proteins of the vertebrate genome is a major next step in the post-genome era. The approximately 4,000 co-translationally translocated (CTT) proteins - representing the vertebrate secretome - are important for such vertebrate-critical processes as organogenesis. However, the role(s) for most of these genes is currently unknown.

RESULTS:

We identified 585 putative full-length zebrafish CTT proteins using cross-species genomic and EST-based comparative sequence analyses. We further investigated 150 of these genes (Figure 1) for unique function using morpholino-based analysis in zebrafish embryos. 12% of the CTT protein-deficient embryos resulted in specific developmental defects, a notably higher rate of gene function annotation than the 2%-3% estimate from random gene mutagenesis studies.

CONCLUSION:

This initial collection includes novel genes required for the development of vascular, hematopoietic, pigmentation, and craniofacial tissues, as well as lipid metabolism, and organogenesis. This study provides a framework utilizing zebrafish for the systematic assignment of biological function in a vertebrate genome.

PMID:
17218990
PMCID:
PMC1766371
DOI:
10.1371/journal.pone.0000104
[Indexed for MEDLINE]
Free PMC Article
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