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Biochem Cell Biol. 2006 Dec;84(6):825-31.

Investigating membrane protein dynamics in living cells.

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1
Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montréal, QC H3G 1Y6, Canada. ian.bates@mcgill.ca

Abstract

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

PMID:
17215870
DOI:
10.1139/o06-189
[Indexed for MEDLINE]
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