Abstract
Endothelial cell barrier dysfunction results in the increased vascular permeability observed in inflammation, tumor metastasis, angiogenesis, and atherosclerosis. Sphingosine 1-phosphate (S1P), a biologically active phosphorylated lipid growth factor released from activated platelets, enhances the endothelial cell barrier integrity in vitro and in vivo. To begin to identify the molecular mechanisms mediating S1P induced endothelial barrier enhancement, quantitative proteomics analysis (iTRAQ) was performed on membrane rafts isolated from human pulmonary artery endothelial cells in the absence or presence of S1P stimulation. Our results demonstrated that S1P mediates rapid and specific recruitment (1 microM, 5 min) of myristoylated alanine-rich protein kinase C substrate (MARCKS) and MARCKS-related protein (MRP) to membrane rafts. Western blot experiments confirmed these findings with both MARCKS and MRP. Finally, small interfering RNA-mediated silencing of MARCKS or MRP or both attenuates S1P-mediated endothelial cell barrier enhancement. These data suggest the regulation of S1P-mediated endothelial cell barrier enhancement via the cell specific localization of MARCKS and MRP and validate the utility of proteomics approaches in the identification of novel molecular targets.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Calmodulin-Binding Proteins
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Capillary Permeability / drug effects
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Capillary Permeability / physiology
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Caveolin 1 / metabolism
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DNA / genetics
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Endothelial Cells / drug effects
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Endothelial Cells / metabolism*
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Humans
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In Vitro Techniques
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Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Lysophospholipids / pharmacology
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Membrane Microdomains / drug effects
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Membrane Microdomains / metabolism*
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Microfilament Proteins
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Molecular Sequence Data
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Myristoylated Alanine-Rich C Kinase Substrate
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Proteomics
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RNA Interference
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RNA, Small Interfering / genetics
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Sphingosine / analogs & derivatives
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Sphingosine / pharmacology
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Tandem Mass Spectrometry
Substances
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CAV1 protein, human
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Calmodulin-Binding Proteins
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Caveolin 1
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Intracellular Signaling Peptides and Proteins
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Lysophospholipids
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MARCKS protein, human
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MARCKSL1 protein, human
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Membrane Proteins
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Microfilament Proteins
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RNA, Small Interfering
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Myristoylated Alanine-Rich C Kinase Substrate
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sphingosine 1-phosphate
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DNA
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Sphingosine