Ethylene responsive element binding protein 1 (StEREBP1) from Solanum tuberosum increases tolerance to abiotic stress in transgenic potato plants

Hye Eun Lee; Dongjin Shin; Sang Ryeol Park; Sang-Eun Han; Mi-Jeong Jeong; Tack-Ryun Kwon; Seong-Kon Lee; Soo-Chul Park; Bu Young Yi; Hawk-Bin Kwon; Myung-Ok Byun

doi:10.1016/j.bbrc.2006.12.095

Ethylene responsive element binding protein 1 (StEREBP1) from Solanum tuberosum increases tolerance to abiotic stress in transgenic potato plants

Biochem Biophys Res Commun. 2007 Feb 23;353(4):863-8. doi: 10.1016/j.bbrc.2006.12.095. Epub 2006 Dec 22.

Authors

Hye Eun Lee¹, Dongjin Shin, Sang Ryeol Park, Sang-Eun Han, Mi-Jeong Jeong, Tack-Ryun Kwon, Seong-Kon Lee, Soo-Chul Park, Bu Young Yi, Hawk-Bin Kwon, Myung-Ok Byun

Affiliation

¹ Postharvest Technology Division, National Horticulture Research Institute, RDA, Suwon 440-706, Republic of Korea.

PMID: 17207469
DOI: 10.1016/j.bbrc.2006.12.095

Abstract

To identify components of the plant stress signal transduction cascade and response mechanisms, we screened plant genes using reverse Northern blot analysis, and chose the ethylene responsive element binding protein 1 (StEREBP1) for further characterization. To investigate its biological function in the potato, we performed Northern blot analysis and observed enhanced levels of transcription in response to several environmental stresses including low temperature. In vivo targeting experiments using a green fluorescent protein (GFP) reporter indicated that StEREBP1 localized to the nucleus of onion epidermal cells. StEREBP1 was found to bind to GCC and DRE/CRT cis-elements and both microarray and RT-PCR analyses indicated that overexpression of StEREBP1 induced expression of several GCC box-containing stress response genes. In addition, overexpression of StEREBP1 enhanced tolerance to cold and salt stress in transgenic potato plants. The results of this study suggest that StEREBP1 is a functional transcription factor that may be involved in abiotic stress responses in plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptation, Physiological / drug effects
Adaptation, Physiological / genetics*
Adaptation, Physiological / physiology
Blotting, Northern
Cell Nucleus / metabolism
Cold Temperature
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Plant / drug effects
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Oligodeoxyribonucleotides / genetics
Oligodeoxyribonucleotides / metabolism
Oligonucleotide Array Sequence Analysis
Plant Proteins / genetics*
Plant Proteins / metabolism
Plants, Genetically Modified
Protein Binding
RNA, Plant / genetics
RNA, Plant / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sodium Chloride / pharmacology
Solanum tuberosum / genetics*
Solanum tuberosum / growth & development
Solanum tuberosum / metabolism

Substances

DNA-Binding Proteins
Oligodeoxyribonucleotides
Plant Proteins
RNA, Plant
Recombinant Fusion Proteins
Green Fluorescent Proteins
ethylene-responsive element binding protein
Sodium Chloride