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Inflamm Bowel Dis. 2007 Feb;13(2):219-24.

Blockade of colony stimulating factor-1 (CSF-I) leads to inhibition of DSS-induced colitis.

Author information

1
Celltech Centre of Excellence for Antibody Research, UCB, 216 Bath Road, Slough SLI 4EN, UK. diane.marshall@celltech.ucb-group.com

Abstract

BACKGROUND:

Intestinal inflammation associated with inflammatory bowel disease (IBD) is typically characterized by an inflammatory cell infiltrate and pro-inflammatory cytokine production. Of particular interest, the frequency of colony stimulating factor-1 (CSF-l)-expressing cells is increased in active lesions. In this study, we have investigated the role of CSF-1 in mucosal inflammation, using a murine model of colitis induced by dextran sulfate sodium (DSS).

METHODS:

A neutralizing anti-CSF-1 antibody was administered to Balb/c mice that received DSS in their drinking water. Signs of colitis, such as clinical disease score, cellular infiltrate, and cytokine production, were assessed.

RESULTS:

Administration of a neutralizing anti-CSF-1 antibody significantly inhibited DSS-induced colitis. Clinical symptoms, such as weight loss and the appearance of diarrhea or fecal blood, were reduced by CSF-1 blockade; histologic scores were also improved. The cellular infiltrate of macrophages and T cells was inhibited and a trend toward reduced production of pro-inflammatory cytokines was noted.

CONCLUSIONS:

This is the first study to demonstrate that CSF-1 plays an important role in mediating intestinal mucosal inflammation and therefore may prove to be an attractive therapeutic target for intestinal diseases such as inflammatory bowel disease.

PMID:
17206685
DOI:
10.1002/ibd.20055
[Indexed for MEDLINE]

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