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Genome Biol. 2007;8(1):R2.

Normalization of boutique two-color microarrays with a high proportion of differentially expressed probes.

Author information

1
Walter and Eliza Hall Institute of Medical Research, Royal Parade, Parkville, Victoria, Australia. oshlack@wehi.edu.au

Abstract

Normalization is critical for removing systematic variation from microarray data. For two-color microarray platforms, intensity-dependent lowess normalization is commonly used to correct relative gene expression values for biases. Here we outline a normalization method for use when the assumptions of lowess normalization fail. Specifically, this can occur when specialized boutique arrays are constructed that contain a subset of genes selected to test particular biological functions.

PMID:
17204140
PMCID:
PMC1839120
DOI:
10.1186/gb-2007-8-1-r2
[Indexed for MEDLINE]
Free PMC Article

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