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Proteomics. 2007 Feb;7(3):364-6.

MascotDatfile: an open-source library to fully parse and analyse MASCOT MS/MS search results.

Author information

1
Department of Biochemistry and Medical Protein Research, Faculty of Medicine and Health Sciences, Flanders Interuniversity Institute for Biotechnology, Ghent University, Ghent, Belgium.

Abstract

MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT results into an easily accessible and fully functional object model (http://genesis.ugent.be/MascotDatfile). Several scripts based on this library are provided as examples, allowing direct automation of important routine tasks involved in processing peptide identifications.

PMID:
17203510
DOI:
10.1002/pmic.200600682
[Indexed for MEDLINE]

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