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EMBO J. 2007 Jan 24;26(2):301-12. Epub 2007 Jan 4.

Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic.

Author information

1
Biozentrum, University of Basel, Klingelbergstrasse, Basel, Switzerland.

Abstract

The small rab-GTPase RAB-7 acts in endosome and endosome to lysosome traffic. We identified SAND-1 as a protein required for RAB-7 function based on similarities between SAND-1 and RAB-7 RNAi phenotypes. Although the initial uptake of yolk protein in oocytes, or of soluble secreted (ss) GFP in coelomocytes, appeared normal, further transport along the endocytic traffic route was delayed in the absence of SAND-1 function, and yolk proteins failed to reach yolk granules efficiently. Moreover, in coelomocytes, ssGFP and BSA-Texas-Red were endocytosed but not transported to lysosomes. We show that SAND-1 is essential for RAB-7 function at the transition from early to late endosomes, but not for RAB-7 function at lysosomes.

PMID:
17203072
PMCID:
PMC1783445
DOI:
10.1038/sj.emboj.7601498
[Indexed for MEDLINE]
Free PMC Article

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