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J Biol Chem. 2007 Mar 2;282(9):6661-7. Epub 2007 Jan 2.

Heparin binding induces a conformational change in pigment epithelium-derived factor.

Author information

1
Center for Insoluble Protein Structures (inSPIN), Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark.

Abstract

Pigment epithelium-derived factor (PEDF) is a noninhibitory serpin found in plasma and in the extracellular space. The protein is involved in different biological processes including cell differentiation and survival. In addition, it is a potent inhibitor of angiogenesis. The function is likely associated with binding to cell surface receptors in a heparin-dependent way (Alberdi, E. M., Weldon, J. E., and Becerra, S. P. (2003) BMC Biochem. 4, 1). We have investigated the structural basis for this observation and show that heparin induces a conformational change in the vicinity of Lys(178). This structural change was evident both when binding to intact heparin and specific heparin-derived oligosaccharides at physiological conditions or simply when exposing PEDF to low ionic strength. Binding to other glycosaminoglycans, heparin-derived oligosaccharides smaller than hexadecasaccharides (dp16), or type I collagen did not affect the structure of PEDF. The conformational change is likely to expose the epitope involved in binding to the receptor and thus regulates the interactions with cell surface receptors.

PMID:
17202143
DOI:
10.1074/jbc.M610471200
[Indexed for MEDLINE]
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