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J Chromatogr A. 2007 Mar 9;1144(1):30-9. Epub 2006 Dec 27.

Resin characterization by electro-acoustic measurements.

Author information

1
Tosoh Bioscience GmbH, Zettachring 6, 70567 Stuttgart, Germany. egbert.mueller@tosohbioscience.com

Abstract

The electro-acoustic effects, namely the ion vibration potential (IVP) and the colloidal vibration current (CVI), colloidal vibration potential (CVP) first described by P. Debye [P. Debye, J. Chem. Phys. 1 (1933) 13], are a result of charge separation of bound or free ions at different degrees by ultrasonic waves. Today commercial instruments are available to investigate liquid homogeneous and heterogeneous systems. In the present paper the application of this technique for the characterization of salts, protein solutions and resins for biochromatography is shown and valuable information about resins can be derived in a short time. Various resins were investigated with the following results: (1) the CVI magnitude is dependent of several parameters (such as particle size distribution, volume fraction, density difference); (2) the CVI is influenced by the surface modification of the resins. Polymeric modifications decrease the value of CVI. The CVI is generally lower for high capacity resins; (3) the measurement of the electro-acoustic effects can be used to detect small changes in resins. The CVI is dependent of the amount of adsorbed protein in "native" and denatured state.

PMID:
17194462
DOI:
10.1016/j.chroma.2006.11.103
[Indexed for MEDLINE]

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