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J Microbiol Methods. 2007 Mar;68(3):639-42. Epub 2006 Dec 26.

Fluorescent measurement of microalgal neutral lipids.

Author information

1
Hawaii Natural Energy Institute, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1680 East-West Road, POST 109, Honolulu, Hawaii 96822, United States.

Abstract

Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility.

PMID:
17189655
DOI:
10.1016/j.mimet.2006.11.008
[Indexed for MEDLINE]

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