Abstract
Purpose:
To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species.
Design:
Interventional case report.
Methods:
The medical records of a patient presenting with an infectious keratitis were reviewed.
Results:
A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring.
Conclusions:
Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
MeSH terms
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Adult
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Anti-Infective Agents / therapeutic use
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Bacterial Typing Techniques
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Base Sequence
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DNA, Bacterial / analysis*
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Eye Infections, Bacterial / diagnosis
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Eye Infections, Bacterial / drug therapy
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Eye Infections, Bacterial / microbiology*
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Humans
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Keratitis / diagnosis
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Keratitis / drug therapy
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Keratitis / microbiology*
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Male
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Molecular Sequence Data
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Nocardia / genetics*
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Nocardia / isolation & purification
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Nocardia Infections / diagnosis
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Nocardia Infections / drug therapy
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Nocardia Infections / microbiology*
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Ophthalmic Solutions / therapeutic use
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Sequence Analysis, DNA / methods*
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Trimethoprim, Sulfamethoxazole Drug Combination / therapeutic use
Substances
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Anti-Infective Agents
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DNA, Bacterial
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Ophthalmic Solutions
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Trimethoprim, Sulfamethoxazole Drug Combination