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Nat Methods. 2007 Jan;4(1):43-5. Epub 2006 Dec 24.

An Escherichia coli expression-based method for heme substitution.

Author information

1
Department of Chemistry, Lawrence Berkeley National Laboratory, University of California, Berkeley, 211 Lewis Hall, Berkeley, California 94720, USA.

Abstract

Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions.

PMID:
17187078
DOI:
10.1038/nmeth984
[Indexed for MEDLINE]

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