Format

Send to

Choose Destination
Cell Death Differ. 1996 Apr;3(2):199-206.

Isolation, differential splicing and protein expression of a DNase on the human X chromosome.

Author information

1
Deutsches Krebsforschungszentrum, Abteilung Molekulare Genomanalyse, INF 280, Heidelberg, Germany.

Abstract

A systematic search for genes differentially expressed in human tissues resulted in the isolation of a gene encoding a protein with high homology to DNase I. In addition to the recently described cDNA sequence (Parrish et al., 1995) we have isolated a transcript, alternatively spliced in the 5' noncoding region. The gene is located between the QM and the XAP-2 gene in Xq28 and encodes a 302 amino acid protein with 39% identity to human DNase I. Besides a high homology at the nucleotide and amino acid level, most exon-intron boundaries of DNase I and DNase X are identical, indicating that both genes may have evolved from a common ancestor. The predicted function was verified by expression of a recombinant protein in an inducible bacterial system and detection of DNase activity. In contrast to DNase I a 18 kdal amino terminal fragment of the full length 35 kdal protein exhibited DNase activity.

PMID:
17180083

Supplemental Content

Loading ...
Support Center