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Nat Methods. 2007 Feb;4(2):135-7. Epub 2006 Dec 17.

Sensitive protein detection via triple-binder proximity ligation assays.

Author information

1
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Se-75185 Uppsala, Sweden. edith.schallmeiner@genpat.uu.se

Abstract

The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA.

PMID:
17179939
DOI:
10.1038/nmeth974
[Indexed for MEDLINE]

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