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Nat Methods. 2007 Jan;4(1):47-9. Epub 2006 Dec 10.

Retrograde neuronal tracing with a deletion-mutant rabies virus.

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1
Systems Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd., La Jolla, California 92037, USA. iwickersham@salk.edu

Abstract

We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology and physiology of neurons projecting to injection sites within the mammalian brain. The virus cannot spread beyond initially infected cells yet, unlike other viral vectors, replicates its core within them. The cells therefore fluoresce intensely, revealing fine dendritic and axonal structure with no background from partially or faintly labeled cells.

PMID:
17179932
PMCID:
PMC2755236
DOI:
10.1038/nmeth999
[Indexed for MEDLINE]
Free PMC Article
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