Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Biotechnol. 2006 Oct;34(2):225-37.

Large-scale transfection of mammalian cells for the fast production of recombinant protein.

Author information

  • 1Laboratoire de Biotechnologie Vétérinaire et Alimentaire, Faculté de Médecine Vétérinaire, Université de Montréal, CP5000, Sainte-Hyacinthe (Québec) J2S 7C6, Canada.

Abstract

Recombinant proteins (r-proteins) are increasingly important in fundamental research and for clinical applications. As many of these r-proteins are of human or animal origin, cultivated mammalian cells are the host of choice to ensure their functional folding and proper posttranslational modifications. Large-scale transfection of human embryonic kidney 293 or Chinese hamster ovary cells is now an established technology that can be used in the production of hundreds of milligram to gram quantities of a r-protein in less than 1 mo from cloning of its cDNA. This chapter aims to provide an overview of large-scale transfection technology with a particular emphasis on calcium phosphate and polyethylenimine-mediated gene transfer.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk