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J Virol. 1991 Oct;65(10):5579-83.

The flavivirus envelope protein E: isolation of a soluble form from tick-borne encephalitis virus and its crystallization.

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1
Institute of Virology, University of Vienna, Austria.

Abstract

By the use of limited trypsin digestion of purified virions, we generated a membrane anchor-free and crystallizable form of the tick-borne encephalitis virus envelope glycoprotein E. It retained its reactivity with a panel of monoclonal antibodies, and only subtle structural differences from the native protein E were recognized. Treatment with the bifunctional cross-linker dimethylsuberimidate resulted in the formation of a dimer. Crystallization experiments yielded hexagonal rod-shaped crystals suitable for X-ray diffraction analysis.

PMID:
1716695
PMCID:
PMC249068
[Indexed for MEDLINE]
Free PMC Article
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