Format

Send to

Choose Destination
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):303-9. Epub 2006 Dec 12.

Highly sensitive assay for the measurement of serotonin in microdialysates using capillary high-performance liquid chromatography with electrochemical detection.

Author information

1
NEUROCHEM, Institut Fédératif des Neurosciences de Lyon, Plate-forme de Physiologie, Université Lyon 1, France. sparrot@sante.univ-lyon1.fr

Abstract

A highly sensitive isocratic capillary high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) for the simultaneous measurement of serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA) in microdialysates has been developed using a 0.5 mm i.d. capillary column and a 11-nL detection cell. This method, validated on both pharmacological and analytical bases, can be performed using injection volumes as low as 1 microL. The limits of detection were 5.6 x 10(-11)mol/L and 3.0 x 10(-9)mol/L for 5-HT and 5-HIAA. Several applications of the present method are given on microdialysates from rodent brain and human spinal cord.

PMID:
17166782
DOI:
10.1016/j.jchromb.2006.11.045
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center