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J Clin Endocrinol Metab. 2007 Mar;92(3):1023-33. Epub 2006 Dec 12.

Relationship between adipocyte size and adipokine expression and secretion.

Author information

1
Else Kröner-Fresenius-Centre for Nutritional Medicine, Technical University Munich, Am Forum 5, D-85350 Freising-Weihenstephan, Germany. nutritional.medicine@wzw.tum.de

Abstract

CONTEXT:

Adipocytes are known to release a variety of factors that may contribute to the proinflammatory state characteristic for obesity. This secretory function is considered to provide the basis for obesity-related complications such as type 2 diabetes and atherosclerosis.

OBJECTIVE:

To get a better insight into possible underlying mechanisms, we investigated the effect of adipocyte size on adipokine production and secretion.

DESIGN, PATIENTS, AND MAIN OUTCOME MEASURES:

Protein secretion and mRNA expression in cultured adipocytes separated according to cell size from 30 individuals undergoing elective plastic surgery were investigated.

RESULTS:

The mean adipocyte volume of the four fractions ranged from 205 +/- 146 to 1.077 +/- 471 pl. There were strong linear correlations for the secretion of adipokines over time. Secretion of leptin, IL-6, IL-8, TNF-alpha, monocyte chemoattractant protein-1, interferon-gamma-inducible protein 10, macrophage inflammatory protein-1beta, granulocyte colony stimulating factor, IL-1ra, and adiponectin was positively correlated with cell size. After correction for cell surface, there was still a significant difference between fraction IV (very large) and fraction I (small cells), for leptin, IL-6, IL-8, monocyte chemoattractant protein-1, and granulocyte colony-stimulating factor. In contrast, antiinflammatory factors such as IL-1ra and adiponectin lost their association after correction for cell surface area comparing fraction I and IV. In addition, there was a decrease of IL-10 secretion with increasing cell size.

CONCLUSIONS:

The results clearly suggest that adipocyte size is an important determinant of adipokine secretion. There seems to be a differential expression of pro- and antiinflammatory factors with increasing adipocyte size resulting in a shift toward dominance of proinflammatory adipokines largely as a result of a dysregulation of hypertrophic, very large cells.

PMID:
17164304
DOI:
10.1210/jc.2006-1055
[Indexed for MEDLINE]
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