Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2006;420:37-49.

Feeder-free culture of human embryonic stem cells.

Author information

  • 1Department of Obstetrics and Gynecology, Rambam Medical Center, Sohnis and Forman Families Stem Cell Center, Technion-Israel Institute of Technology, Haifa, Israel.

Abstract

In addition to their contribution to research fields such as early human development, self-renewal, and differentiation mechanisms, human embryonic stem cells (hESCs) may serve as a tool for drug testing and for the study of cell-based therapies. Traditionally, these cells have been cultured with mouse embryonic fibroblast (MEF) feeder layers, which allow their continuous growth in an undifferentiated state. However, for future clinical applications, hESCs should be cultured under defined conditions, preferably in a xeno-free culture system, where exposure to animal pathogens is prevented. To this end, different culture methods for hESCs, based on serum replacement and free of supportive cell layers, were developed. This chapter discusses a simple, feeder-free culture system on the basis of medium supplemented with transforming growth factor beta1 (TGFbeta1), basic fibroblast growth factor (bFGF) and fibronectin as matrix.

PMID:
17161692
DOI:
10.1016/S0076-6879(06)20003-X
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center