Metaproteomics approach to study the functionality of the microbiota in the human infant gastrointestinal tract

Eline S Klaassens; Willem M de Vos; Elaine E Vaughan

doi:10.1128/AEM.01921-06

Metaproteomics approach to study the functionality of the microbiota in the human infant gastrointestinal tract

Appl Environ Microbiol. 2007 Feb;73(4):1388-92. doi: 10.1128/AEM.01921-06. Epub 2006 Dec 8.

Authors

Eline S Klaassens¹, Willem M de Vos, Elaine E Vaughan

Affiliation

¹ Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Eline.klaassens@wur.nl

Abstract

A metaproteomics approach comprising two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) was applied to the largely uncultured infant fecal microbiota for the first time. The fecal microbial metaproteome profiles changed over time, and one protein spot contained a peptide sequence that showed high similarity to those of bifidobacterial transaldolases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Gel, Two-Dimensional
Gastrointestinal Tract / microbiology*
Humans
Infant
Molecular Sequence Data
Peptides / physiology
Proteome / physiology*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Transaldolase / metabolism

Substances

Peptides
Proteome
Transaldolase

Associated data

GENBANK/DQ323457
GENBANK/DQ323458
GENBANK/DQ323459