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Genome Biol. 2006;7(12):R116.

Generation of medaka gene knockout models by target-selected mutagenesis.

Author information

1
Department of Radiation Genetics, CREST, Japan Science and Technology Laboratory, Kyoto University, Yoshida Konoe, Sakyo-ku, Kyoto 606-8501, Japan. .

Abstract

We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

PMID:
17156454
PMCID:
PMC1794429
DOI:
10.1186/gb-2006-7-12-r116
[Indexed for MEDLINE]
Free PMC Article

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